Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Helicase

Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Helicase

Middle East respiratory syndrome coronavirus (MERS-CoV) helicase is a superfamily 1 helicase containing seven conserved motifs. We have cloned, expressed, and purified a Strep-fused recombinant MERS-CoV nonstructural protein 13 (M-nsp13) helicase. Characterization of its biochemical properties showed that it unwound DNA and RNA similarly to severe acute respiratory syndrome CoV nsp13 (S-nsp13) ...

Crystal structure of Middle East respiratory syndrome coronavirus helicase

Middle East respiratory syndrome coronavirus (MERS-CoV) remains a threat to public health worldwide; however, effective vaccine or drug against CoVs remains unavailable. CoV helicase is one of the three evolutionary most conserved proteins in nidoviruses, thus making it an important target for drug development. We report here the first structure of full-length coronavirus helicase, MERS-CoV nsp...

Middle East respiratory syndrome coronavirus

International experts were invited by WHO to join an international team, which visited Riyadh 4-9 June 2013. The joint Saudi/WHO team comprised officials from the Ministry of Health (MoH) of the Kingdom of Saudi Arabia and experts from the Food and Agriculture Organization of the United Nations (FAO), the World Organisation for Animal Health (OIE) and public health institutions and hospitals in...

middle east respiratory syndrome coronavirus

Middle East respiratory syndrome coronavirus

International experts were invited by WHO to join an international team, which visited Riyadh 4-9 June 2013. The joint Saudi/WHO team comprised officials from the Ministry of Health (MoH) of the Kingdom of Saudi Arabia and experts from the Food and Agriculture Organization of the United Nations (FAO), the World Organisation for Animal Health (OIE) and public health institutions and hospitals in...